Protein structure, stability, and interactions / ed. by J.W.Shriver. - New York: Humana, 2009. - x, 359 p.: ill. - (Springer protocols. Methods in molecular biology; 490). - Incl. bibl. ref. - Ind.: p.353-359. - ISBN 978-1-58829-954-3; ISSN 1064-3745  Место хранения:   056 | Институт химической биологии и фундаментальной медицины CO РАН | Новосибирск| Библиотека

Оглавление / Contents

Preface ......................................................... v Contributors ................................................... ix 1 Microcalorimetry of Proteins and Their Complexes ............. 7 Peter L. Privalov 2 Determining the Conformational Stability of a Protein Using Urea Denaturation Curves .............................. 47 Kevin L. Shaw, J. Martin Scholtz, C. Nick Pace, and Gerald R. Grimsley 3 Defining the Stability of Multimeric Proteins ............... 57 John W. Shriver, and Stephen P. Edmondson 4 Protein-Protein and Ligand-Protein Interactions Studied by Analytical Ultracentrifugation .............................. 83 Walter F. Stafford, III 5 Monitoring Molecular Interactions by NMR ................... 115 James M. Lipchock, and J. Patrick Loria 6 Ligand-Binding Interactions and Stability .................. 135 John W. Shriver, and Stephen P. Edmondson 7 A Method for Direct Measurement of Protein Stability In Vivo ....................................................... 765 Zoya Ignatova, and Lila M. Gierasch 8 Quantifying the Roles of Water and Solutes (Denaturants, Osmolytes, and Hofmeister Salts) in Protein and Model Processes Using the Solute Partitioning Mode ............... 779 Laurel M. Pegram, and M. Thomas Record, Jr. 9 Molecular Crowding and Solvation: Direct and Indirect Impact on Protein Reactions ................................ 795 Jörg Rösgen 10 Defining the Role of Salt Bridges in Protein Stability ..... 227 Ilian Jelesarov, and Andrey Karshikoff 11 Protein Stabilization by the Rational Design of Surface Charge-Charge Interactions ................................. 267 Katrina L. Schweiker, and George I. Makhatadze 12 NMR Analysis of Native-State Protein Conformational Flexibility by Hydrogen Exchange ........................... 285 Griselda Hernández, and David M. LeMaster 13 Single-Molecule Fluorescence Studies of Protein Folding .... 377 G. Ulrich Nienhaus 14 Experimental Characterization of the Denatured State Ensemble of Proteins ....................................... 339 Jae-Hyun Cho, and Daniel P. Raleigh Index ......................................................... 353