Chapter 1
1 General Introduction ......................................... 1
1.1 Bioreporter bacteria .................................... 4
1.1.1 The genetic principle ............................ 4
1.1.2 The application in environmental research ........ 6
1.1.3 Comparison with analytical chemistry ............. 8
1.2 Arsenic ................................................ 10
1.2.1 Occurrence in the environment ................... 10
1.2.2 Arsenic metabolism in bacteria and plants ....... 12
1.2.3 Arsenic as target for bioreporter bacteria ...... 14
1.3 Outline of the thesis .................................. 15
References .................................................. 17
Chapter 2
2 Internal arsenite bioassay calibration using multiple
bioreporter cell lines ...................................... 24
Chapter 3
3 Optimization of preservation conditions of As (III)
bioreporter bacteria ........................................ 34
Chapter 4
4 Visualisation of gradients in arsenic concentrations
around individual roots of Zea mays L. using agar-
immobilized bioreporter bacteria ............................ 43
Chapter 5
5 Summary and concluding remarks .............................. 75
5.1 Bioreporter bacteria in drinking water analysis ........ 76
5.2 Bioreporter bacteria for arsenic in situ detection in
the rhizosphere ........................................ 78
5.3 Concluding remarks about bioreporter bacteria, their
limits and perspectives ................................ 79
References ..................................................... 83
Acknowledgements ............................................... 85
Publications ................................................... 86
Curriculum Vitae ............................................... 87
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