Genotoxicity assessment: methods and protocols / ed. by A.Dhawan, M.Bajpayee. - New York: Humana press/Springer Science+Business Media, 2013. - xv, 463 p.: ill. - (Methods in molecular biology; 1044) (Springer protocols). - Bibliogr. at the end of the chapters. - Ind.: p.459-463. - ISBN 978-1-62703-528-6ж ISSN 1064-3745  Место хранения:   02 | Отделение ГПНТБ СО РАН | Новосибирск

Оглавление / Contents

Preface ....................................................... vii Acknowledgements ............................................... ix Contributors ................................................. xiii Part I Gene Mutation Assays 1 Bacterial Mutation Assays .................................... 3 Errol Zeiger 2 In Vitro Mouse Lymphoma (L5178Y Tk+/- -3.7.2C) Forward Mutation Assay .............................................. 27 Melissa R. Schisler, Martha M. Moore, and B. Bhaskar Gollapudi 3 Erythrocyte-Based Pig-α Gene Mutation Assay ................. 51 Jeffrey C. Bemis, Nikki E. Hall, and Stephen D. Dertinger 4 Detection of In Vivo Mutation in the Hprt and Pig-α Genes of Rat Lymphocytes .......................................... 79 Vasily N. Dobrovolsky, Joseph G. Shaddock, Roberta A. Mittelstaedt, Daishiro Miura, and Robert H. Heflich 5 In Vivo ell, gpt, and Spi Gene Mutation Assays in Transgenic Mice and Rats ................................. 97 Mugimane G. Manjanatha, Xuefei Cao, Sharon D. Shelton, Roberta A. Mittelstaedt, and Robert H. Heflich Part II Assays for Chromosomal Abnormalities 6 In Vitro Cytogenetic Assays: Chromosomal Aberrations and Micronucleus Tests ......................................... 123 Pasquale Mosesso, Serena Cinelli, Adyapalam T. Natarajan, and Fabrizio Palitti 7 Analysis of Chromosome Aberrations in Somatic and Germ Cells of the Mouse ......................................... 147 Francesca Pacchierotti and Valentina Stocchi 8 Chromosomal Aberration Test in Human Lymphocytes ........... 165 Christian Johannes and Guenter Obe 9 In Vivo Micronucleus Assay in Mouse Bone Marrow and Peripheral Blood ........................................... 179 Sawako Kasamoto, Shoji Masumori, and Makoto Hayashi 10 Micronucleus Assay in Human Cells: Lymphocytes and Buccal Cells ...................................................... 191 Claudia Bolognesi and Michael Fenech 11 Flow Cytometric Determination of Micronucleus Frequency .... 209 Azeddine Elhajouji and Magdalena Lukamowicz-Rajska 12 Fluorescence In Situ Hybridization (FISH) Technique for the Micronucleus Test .................................. 237 Use Decordier and Micheline Kirsch-Volders 13 Comparative Genomic Hybridization (CGH) in Genotoxicology .. 245 Adolf Baumgartner 14 The In Vitro Micronucleus Assay and Kinetochore Staining: Methodology and Criteria for the Accurate Assessment of Genotoxicity and Cytotoxicity ........................... 269 Bella B. Manshian, Neenu Singh, and Share en H. Doak Part III Tests for Primary DNA Damage 15 γ-H2AX Detection in Somatic and Germ Cells of Mice ......... 293 Eugenia Cordelli and Lorena Paris 16 Multicolor Laser Scanning Confocal Immunofluorescence Microscopy of DNA Damage Response Biomarkers ............... 311 Julian Laubenthal, Michal R. Gdula, Alok Dhawan, and Diana Anderson 17 The Comet Assay: Assessment of In Vitro and In Vivo DNA Damage ..................................................... 325 Mahima Bajpayee, Ashutosh Kumar, and Alok Dhawan 18 The Comet Assay in Human Biomonitoring ..................... 347 Diana Anderson, Alok Dhawan, and Julian Laubenthal 19 The Comet Assay in Marine Animals .......................... 363 Giada Frenzilli and Brett P. Lyons 20 Unscheduled DNA Synthesis (UDS) Test with Mammalian Liver Cells In Vivo .............................................. 373 Fabrice Nesslany 21 32P-Postlabeling Analysis of DNA Adducts ................... 389 Heinz H. Schmeiser, Marie Stiborova, and Volker M. Arlt Part IV Assays in Plants and Alternate Animal Models 22 Micronucleus Assay with Tetrad Cells of Tradescantia ....... 405 Miroslav Mišík, Clemens Pichler, Bernhard Rainer, Armen Nersesyan, and Siegfried Knasmueller 23 The Wing-Spot and the Comet Tests as Useful Assays Detecting Genotoxicity in Drosophila ....................... 417 Ricard Marcos and Erico R. Carmona Part V Guidelines 24 Genotoxicity Guidelines Recommended by International Conference of Harmonization (ICH) .......................... 431 Gireesh H. Kamath and K.S. Rao Index ......................................................... 459