Preface ......................................................... v
Contributors ................................................... ix
1 Transgenic Mouse Models ...................................... 1
Jennifer Skelton, Linda H. Gower, Anne McGough, and
Patricia A. Labosky
2 Pulmonary Antigen Presenting Cells: Isolation,
Purification, and Culture ................................... 19
Hideki Nakano and Donald N. Cook
3 Evaluation of T Cell Function in Allergic Disease ........... 31
Brianne R. Barker
4 Evaluating B-Cells: From Bone Marrow Precursors to
Antibody-Producing Cells .................................... 45
M. Manuela Rosado, Marco Scarsella, Simona Cascioli, Ezio
Giorda, and Rita Carsetti
5 Protocols for Identifying, Enumerating, and Assessing
Mouse Eosinophils ........................................... 59
Kimberly D. Dyer, Katia E. Garcia-Crespo, Caroline
M. Percopo, Eva M. Sturm, and Helene F. Rosenberg
6 Evaluation of Classical, Alternative, and Regulatory
Functions of Bone Marrow-Derived Macrophages ................ 79
Beckley K. Davis
7 Applications of Mouse Airway Epithelial Cell Culture for
Asthma Research ............................................. 91
Amjad Horani, John D. Dickinson, and Steven L. Brody
8 Isolation and Characterization of Mast Cells in Mouse
Models of Allergic Diseases ................................ 109
Martina Kovarova
9 Purifying and Measuring Immunoglobulin E (IgE) and
Anti-IgE ................................................... 121
Jamie L. Sturgill and Daniel H. Conrad
10 Protocols for the Induction and Evaluation of Systemic
Anaphylaxis in Mice ........................................ 133
Elizabeth Doyle, Julia Trosien, and Martin Metz
11 Contact Hypersensitivity Models in Mice .................... 139
Irving C. Allen
12 Induction of Allergic Rhinitis in Mice ..................... 145
Virginia McMillan Carr and Alan M. Robinson
13 Induction of Allergic Airway Disease Using House Dust
Mite Allergen .............................................. 159
Irving C. Allen
14 An Inhalation Model of Allergic Fungal Asthma:
Aspergillus fumigatus-Induced Inflammation and Remodeling
in Allergic Airway Disease ................................. 173
Jane M. Schuh and Scott A. Hoselton
15 PAMPs and DAMPs in Allergy Exacerbation Models ............. 185
Monique A.M. Willart, Philippe Poulliot, Bart N.
Lambrecht, and Mirjam Kool
16 Assessment of Airway Hyperresponsiveness in Mouse Models
of Allergic Lung Disease Using Detailed Measurements
of Respiratory Mechanics ................................... 205
John M. Hartney and Annette Robichaud
17 Bilateral Vagotomy as a Tool for Determining Autonomic
Involvement in Airway Responses in Mouse Models of
Asthma ..................................................... 219
Jaime M. Cyphert
18 Clara Epithelial Cell Depletion in the Lung ................ 229
Sanchaita S. Sonar and Jan C. Dudda
19 A Mouse Model for Evaluating the Contribution of
Fibrocytes and Myofibroblasts to Airway Remodeling
in Allergic Asthma ......................................... 235
Matthias Schmidt and Sabrina Mattoli
20 Assessment of Airway Hyperresponsiveness in Murine
Tracheal Rings ............................................. 257
Jeremiah T. Herlihy, Iurii Semenov, and Robert Brenner
21 Use of the Cockroach Antigen Model of Acute Asthma to
Determine the Immunomodulatory Role of Early
Exposure to Gastrointestinal Infection ..................... 271
Carolyn G. Durham, Lisa M. Schwiebert, and Robin
G. Lorenz
22 Expression Profiling to Identify Candidate Genes
Associated with Allergic Phenotypes ........................ 287
Willie June Brickey
23 Flow Cytometric Methods for the Assessment of Allergic
Disease .................................................... 297
Adeeb H. Rahman
24 Generation of Bone Marrow and Fetal Liver Chimeric Mice .... 315
Eda K. Holl
Index ......................................................... 323
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