Microglia: methods and protocols / ed. by B.Joseph, J.L.Venero. - New York: Humana press/Springer Science+Business Media, 2013. - XII,350 p. : ill. - (Methods in molecular biology; 1041) (Springer protocols). - Bibliogr. at the end of the chapters. - Ind.: p. 347-350. - ISBN 978-1-62703-519-4; ISSN 1064-3745  Место хранения:   02 | Отделение ГПНТБ СО РАН | Новосибирск

Оглавление / Contents

Preface ......................................................... v Contributors ................................................... xi Part I Overview of Microglia Biology 1 A Brief Overview of Multitalented Microglia .................. 3 Bertrand Joseph and Jose Luis Venero Part II Isolation and Culture of Microglia 2 Cell Culturing of Human and Murine Microglia Cell Lines ..... 11 Johanna Rodhe 3 Microglia Isolation from Adult Mouse Brain .................. 17 Jae-Kyung Lee and Malu G. Tansey 4 Preparation of Primary Microglia Cultures from Postnatal Mouse and Rat Brains ........................................ 25 Tomas Deierborg 5 Isolation of Murine Postnatal Brain Microglia for Phenotypic Characterization Using Magnetic Cell Separation Technology .................................................. 33 Ashley S. Harms and Malú G. Tansey 6 Isolation and Culture of Adult Human Microglia Within Mixed Glial Cultures for Functional Experimentation and High-Content Analysis ....................................... 41 Amy M. Smith, Hannah M. Gibbons, Claire Lill, Richard L.M. Fault, and Mike Dragunow Part III Depletion and Transduction of Microglia 7 Depletion of Microglia from Primary Cellular Cultures ....... 55 Lorena Pont-Lezica, Sabrina Colasse, and Alain Bessis 8 Lentiviral Transduction of Cultured Microglia ............... 63 Takahiro Masuda, Makoto Tsuda, Hidetoshi Tozaki-Saitoh, and Kazuhide Inoue Part IV Analysis of Microglial Cytokine Production 9 Microglial Activation: Measurement of Cytokines by Flow Cytometry ................................................... 71 Deepak Kumar Kaushik and Anirban Basu 10 In Situ Hybridization of Cytokine mRNA Using Alkaline Phosphatase-Labelled Oligodeoxynucleotide Probes ............ 83 Bettina Clausen, Christina Fenger, and Bente Finsen 11 Use of Meso-Scale DiscoveryTM to Examine Cytokine Content in Microglia Cell Supernatant ............................... 93 Miguel A. Burguillos Part V Analysis of Microglia Activation 12 Analysis of Microglial Production of Reactive Oxygen and Nitrogen Species ........................................... 103 Urte Neniskyte and Guy C. Brown 13 Quantification of Active Caspase-3 and Active Caspase-8 in Microglia Cells ......................................... 113 Edel Kavanagh 14 Quantification of Microglial Phagocytosis by a Flow Cytometer-Based Assay ...................................... 121 Refik Pul, Kandiyil Prajeeth Chittappen, and Martin Stangel 15 Quantification of Microglial Proliferation and Apoptosis by Flow Cytometry .......................................... 129 Alicia A. Babcock, Martin Wirenfeldt, and Bente Finsen 16 Fluorescence Imaging of Intracellular Ca2+, Na+, and H+ in Cultured Microglia ...................................... 147 Tom Schilling and Claudia Eder 17 Patch Clamp Protocols to Study Ion Channel Activity in Microglia .................................................. 163 Tom Schilling and Claudia Eder Part VI Analysis of Microglia Polarization 18 Studying M1 and M2 States in Adult Microglia ............... 185 Sadanand M. Gaikwad and Michael T. Heneka 19 Isolating, Culturing, and Polarizing Primary Human Adult and Fetal Microglia ........................................ 199 Bryce A. Durafourt, Craig S. Moore, Manon Blain, and Jack P. Antel Part VII Co-culture Systems to Analysis Microglia Interactions with Other Cell Types 20 Understanding Microglia-Neuron Cross Talk: Relevance of the Microglia-Neuron Cocultures ......................... 215 Fernando G. Correa, Miriam Hernangómez, and Carmen Guaza 21 Preparation of Rodent Primary Cultures for Neuron-Glia, Mixed Glia, Enriched Microglia, and Reconstituted Cultures with Microglia .................................... 231 Shih-Heng Chen, Esteban A. Oyarzabal, and Jau-Shyong Hong Part VIII Analysis of Microglia Functions In Vivo 22 Microglia Detection by Enzymatic Histochemistry ............ 243 Beatriz Almolda, Berta González, and Bernardo Castellano 23 Tomato Lectin Histochemistry for Microglial Visualization .............................................. 261 Nàdia Villacampa, Beatriz Almolda, Berta González, and Bernardo Castellano 24 Immunohistochemical Detection of Microglia ................. 281 Rocío M. de Pablos, Ana M. Espinosa-Oliva, and Antonio J. Herrera 25 Intrathecal Infusion of Microglia Cells .................... 291 Takahiro Masuda, Makoto Tsuda, Hidetoshi Tozaki-Saitoh, and Kazuhide Inoue 26 Intracranial Injection of LPS in Rat as Animal Model of Neuroinflammation ....................................... 295 Ana M. Espinosa-Oliva, Rocio M. de Pablos, and Antonio J. Herrera 27 Analyses of Microglia Effector Function Using CX3CR1-GFP Knock-In Mice .............................................. 307 Jenny A. Garcia, Sandra M. Cardona, and Astrid E. Cardona 28 In Vivo Two-Photon Microscopy of Microglia ................. 319 Satoru Kondo and SMgeo Okabe 29 Use of Confocal Microscopy in the Study of Microglia in a Brain Metastasis Model ................................ 337 Manuel Sarmiento Index ......................................................... 347