 | Serdyuk I.N. Methods in molecular biophysics: structure, dynamics, function / I.N.Serdyuk, N.R.Zaccai, J.Zaccai. - Cambridge; New York: Cambridge univ. press, 2007. - xvi, 1120 p.: ill. - Bibliogr.: p.1076-1102. - Ind.: p.1103-1120. - Пер. загл.: Методы в молекулярной биофизике: структура, динамика, функция. - ISBN 0-521-81524-X
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Foreword by D.M. Engelman ...................................... xi
Foreword by Pierre Joliot .................................... xiii
Preface ........................................................ xv
Introduction Molecular biophysics at the beginning of the
twenty-first century: from ensemble measurements to
single-molecule detection ....................................... 1
Part A Biological macromolecules and physical tools ........... 19
Chapter A1 Macromolecules in their environment ................ 21
Chapter A2 Macromolecules as physical particles ............... 38
Chapter A3 Understanding macromolecular structures ............ 65
Part В Mass spectrometry ..................................... 109
Chapter В1 Mass and charge ................................... 111
Chapter B2 Structure function studies ........................ 136
Part C Thermodynamics ........................................ 171
Chapter C1 Thermodynamic stability and interactions .......... 173
Chapter C2 Differential scanning calorimetry ................. 194
Chapter C3 Isothermal titration calorimetry .................. 221
Chapter C4 Surface plasmon resonance and interferometry-
based biosensors .................................. 234
Part D Hydrodynamics ......................................... 247
Chapter D1 Biological macromolecules as hydrodynamic
particles ......................................... 249
Chapter D2 Fundamental theory ................................ 268
Chapter D3 Macromolecular diffusion .......................... 318
Chapter D4 Analytical ultracentrifugation .................... 339
Chapter D5 Electrophoresis ................................... 388
Chapter D6 Electric birefringence ............................ 414
Chapter D7 Flow birefringence ................................ 435
Chapter D8 Fluorescence depolarisation ....................... 446
Chapter D9 Viscosity ......................................... 466
Chapter D10 Dynamic light scattering .......................... 481
Chapter D11 Fluorescence correlation spectroscopy ............. 505
Part E Optical spectroscopy .................................. 517
Chapter E1 Visible and IR absorption spectroscopy ............ 519
Chapter E2 Two-dimensional IR spectroscopy ................... 562
Chapter E3 Raman scattering spectroscopy ..................... 573
Chapter E4 Optical activity .................................. 601
Part F Optical microscopy .................................... 625
Chapter F1 Light microscopy .................................. 627
Chapter F2 Atomic force microscopy ........................... 641
Chapter F3 Fluorescence microscopy ........................... 658
Chapter F4 Single-molecule detection ......................... 683
Chapter F5 Single-molecule manipulation ...................... 709
Part G X-ray and neutron diffraction ......................... 765
Chapter G1 The macromolecule as a radiation scattering
particle .......................................... 767
Chapter G2 Small-angle scattering ............................ 794
Chapter G3 X-ray and neutron macromolecular crystallography .. 838
Part H Electron diffraction .................................. 883
Chapter H1 Electron microscopy ............................... 885
Chapter H2 Three-dimensional reconstruction from
two-dimensional images ............................ 904
Part I Molecular dynamics .................................... 929
Chapter I1 Energy and time calculations ...................... 931
Chapter I2 Neutron spectroscopy .............................. 948
Part J Nuclear magnetic resonance ............................ 969
Chapter J1 Frequencies and distances ......................... 971
Chapter J2 Experimental techniques .......................... 1000
Chapter J3 Structure and dynamics studies ................... 1039
References ................................................... 1076
Index of eminent scientists .................................. 1103
Subject index ................................................ 1108
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