Synthetic messenger RNA and cell metabolism modulation: methods and protocols / ed. by P.M.Rabinovich. - New York: Humana Press, 2013. - xi, 323 p.: ill. - (Methods in molecular biology; 969) (Springer protocols). - Incl. bibl. ref. - Ind.: p.321-323. - ISBN 978-1-62703-259-9; ISSN 1064-3745  Место хранения:   056 | Институт химической биологии и фундаментальной медицины CO РАН | Новосибирск| Библиотека

Оглавление / Contents

Preface ......................................................... v Contributors ................................................... ix Part I mRNA Synthesis, Purification, and Transfer 1 Cell Engineering with Synthetic Messenger RNA ................ 3 Peter M. Rabinovich and Sherman M. Weissman 2 In Vitro Transcription of Long RNA Containing Modified Nucleosides ................................................. 29 Norbert Pardi, Hiromi Muramatsu, Drew Weissman, and Katalin Karikó 3 HPLC Purification of In Vitro Transcribed Long RNA .......... 43 Drew Weissman, Norbert Pardi, Hiro Muramatsu, and Katalin Karikó 4 Synthetic mRNAs with Superior Translation and Stability Properties .................................................. 55 Ewa Grudzien-Nogalska, Joanna Kowalska, Wei Su, Andreas N. Kuhn, Sergey V. Slepenkov, Edward Darzynkiewicz, Ugur Sahin, Jacek Jemielity, and Robert E. Rhoads 5 Nonviral, Cationic Lipid-Mediated Delivery of mRNA .......... 73 James G. Hecker 6 Light-Induced mRNA Transfection ............................. 89 Sigurd Leinæs Bøe and Eivind Hovig 7 Engineering В Cells with mRNA .............................. 101 Jaewoo Lee, David Boczkowski, and Smita Nair 8 Programming Human Dendritic Cells with mRNA ................ 111 Jaewoo Lee, David Boczkowski, and Smita Nair 9 Large Volume Flow Electroporation of mRNA: Clinical Scale Process .................................................... 127 Linhong Li, Cornell Allen, Rama Shivakumar, and Madhusudan V. Peshwa 10 Retrovirus-Based mRNA Transfer for Transient Cell Manipulation ............................................... 139 Melanie Galla, Axel Schambach, and Christopher Baum 11 mRNA Delivery to Human Dendritic Cells by Recombinant Yeast and Activation of Antigen-Specific Memory T Cells .... 163 Frank Breinig, Tanja Breinig, and Manfred J. Schmitt Part II Modulation and Monitoring of Cell Metabolism 12 Nonviral RNA Transfection to Transiently Modify T Cells with Chimeric Antigen Receptors for Adoptive Therapy ....... 187 Tobias Riet, Astrid Holzinger, Jan Dörrie, Niels Schaft, Gerold Schuler, and Hinrich Abken 13 Natural Killer Cell Reprogramming with Chimeric Immune Receptors .................................................. 203 Noriko Shimasaki and Dario Саmpanа 14 Reprogramming to Pluripotency and Differentiation of Cells with Synthetic mRNA .................................. 221 Peizhe Wang and Jie Na 15 Antitumor Vaccination with Synthetic mRNA: Strategies for In Vitro and In Vivo Preclinical Studies ................... 235 Mustafa Diken, Sebastian Kreiter, Abderraouf Selmi, Özlem Türeci, and Ugur Sahin 16 Mannosylated and Histidylated LPR Technology for Vaccination with Tumor Antigen mRNA ........................ 247 Chantal Pichon and Patrick Midoux 17 Analysis of Survivin-Specific T Cells in Breast Cancer Patients Using Human DCs Engineered with Survivin mRNA ..... 275 Özcan Met and Inge Marie Svane 18 mRNA Electroporation as a Tool for Immunomonitoring ........ 293 Nathalie Cools, Kirsten Van Camp, Viggo Van Tendeloo, and Zwi Berneman 19 mRNA PCR-Based Epitope Chase Method ........................ 305 Jean-Daniel Doucet, Dominique Gauchat, and Réjean Lapointe Index ......................................................... 321