Foreword ........................................................ v
Preface ....................................................... vii
Contributors ................................................. xiii
Section I Reviews .............................................. 1
1 Review of Human DC Subtypes .................................. 3
Xinsheng Ju, Georgina Clark, and Derek N.J. Hart
2 Review of Murine Dendritic Cells: Types, Location, and
Development ................................................. 21
Tewfik Miloud, Günter J. Hämmerling, and Natalio Garbi
Section II Human DC Protocols ................................. 43
3 Isolation of Human Blood DC Subtypes ........................ 45
Andrew J. Kassianos, Sarah L. Jongbloed, Derek N.J. Hart,
and Kristen J. Radford
4 Isolation of Immature Primary Langerhans Cells from Human
Epidermal Skin .............................................. 55
Marein A.W.P. de Jong, Lot de Witte, and Teunis
B.H. Geijtenbeek
5 Isolation and In Vitro Generation of Gene-Manipulated
Human Plasmacytoid and Conventional Dendritic Cells ......... 67
Remko Schotte, Heike Schmidlin, Maho Nagasawa, Wendy
Dontje, Julien J. Karrich, Christel Uittenbogaart, Hergen
Spits, and Bianca Blom
6 In Vivo Modulation of Gene Expression by Lentiviral
Transduction in "Human Immune System" Rag2-/-γc-/- Mice ... 87
Anja U. van Lent, Mireille Centlivre, Maho Nagasawa,
Julien J. Karrich, Stephan M. Pouw, Kees Weijer, Hergen
Spits, Bianca Blom, and Nicolas Legrand
7 Generation of Stable Thl/CTL-, Th2-, and Thl7-Tnducing
Human Dendritic Cells ...................................... 117
Pawel Kalinski, Eva Wieckowski, Ravikumar Muthuswamy, and
Esther de Jong
8 Pharmacological Modification of Dendritic Cells to
Promote Their Tolerogenicity in Transplantation ............ 135
Hēth R. Turnquist, Ryan T. Fischer, and Angus W. Thomson
9 Towards a Standardized Protocol for the Generation
of Monocyte-Derived Dendritic Cell Vaccines ................ 149
Michael Erdmann and Beatrice Schuler-Thurner
Section III Murine Dendritic Cell Methods ..................... 165
10 CD8+, CD8-, and Plasmacytoid Dendritic Cell Generation
In Vitro Using flt3 Ligand ................................. 167
Shalin H. Naik, Meredith O'Keeffe, Anna Proietto,
Hubertus Hochrein Ken Shortman, and Li Wu
11 Generation of Large Numbers of Pro-DCs and Pre-DCs In
Vitro ...................................................... 177
Shalin H. Naik
12 The Generation of Plasmacytoid and Conventional Dendritic
Cells with M-CSF ........................................... 187
Meredith O'Keeffe, Ben Fancke, and Hubertus Hochrein
13 Isolation of Common Dendritic Cell Progenitors (CDP)
from Mouse Bone Marrow ..................................... 195
Nobuyuki Onai, Markus G. Manz, and Michael A. Schmid
14 The Isolation of Mouse Dendritic Cells from Lymphoid
Tissues and the Identification of Dendritic Cell Subtypes
by Multiparameter Flow Cytometry ........................... 205
David Vremec
15 Isolation of Cutaneous Dendritic Cells ..................... 231
Julie Helft and Miriam Merad
16 Isolation of Skin Dendritic Cells from Mouse and Man ....... 235
Patrizia Stoitzner, Nikolaus Romani, Alexander
D. McLellan, Christoph H. Tripp, and Susanne Ebner
17 Identification and Isolation of Rodent Respiratory Tract
Dendritic Cells ............................................ 249
Philip A. Stumbles, Deborah H. Strickland, Mathew
E. Wikstrom, Jennifer A. Thomas, Christophe von Gamier,
and Patrick G. Holt
18 Isolation of Mouse Thymic Dendritic Cell Precursors ........ 265
Li Wu and Angela D'Amico
19 Isolation of Rat Intestinal Lymph DC ....................... 281
Simon Milling and Gordon MacPherson
20 Mouse Models of Viral Infection: Influenza Infection
in the Lung ................................................ 299
Adele M. Mount and Gabrielle T. Belz
21 DCs in Mouse Models of Intracellular Bacterial Infection ... 319
Michael Neuenhahn, Matthias Schiemann, and Dirk H. Busch
22 Studying the Function of Dendritic Cells in Mouse Models
of Asthma .................................................. 331
Philippe Pouliot, Monique A. Willart, Hamida Hammad, and
Bart N. Lambrecht
23 Direct Ex Vivo Activation of T cells for Analysis of
Dendritic Cells Antigen Presentation ....................... 351
Gabrielle T. Belz
24 Methods to Study Pulmonary Dendritic Cell Migration ........ 371
Claudia Jakubzick and Gwendalyn J. Randolph
25 Measuring pH, ROS Production, Maturation, and Degradation
in Dendritic Cell Phagosomes Using Cytofluorometry-Based
Assays ..................................................... 383
Ariel Savina, Pablo Vargas, Pierre Guermonprez, Ana-Maria
Lennon, and Sebastian Amigorena
26 Adaptive Treg Generation by DCs and Their Functional
Analysis ................................................... 403
Li Wang
27 Generation of Parabiotic Mice for the Study of DC and DC
Precursor Circulation ...................................... 413
Claudia Waskow
28 Defining In Vivo Dendritic Cell Functions Using CDllc-DTR
Transgenic Mice ............................................ 429
Liat Bar-On andSteffen Jung
Subject Index ................................................. 443
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