Cell senescence: methods and protocols / ed. by L.Galluzzi, I.Vitale, O.Kepp, G.Kroemer. - New York: Humana Press, 2013. - xii, 538 p.: ill. - (Methods in molecular biology; 965) (Springer protocols). - Incl. bibl. ref. - Ind.: p.533-538. - ISBN 978-1-62703-238-4; ISSN 1064-3745  Место хранения:   056 | Институт химической биологии и фундаментальной медицины CO РАН | Новосибирск| Библиотека

Оглавление / Contents

Preface ......................................................... v Contributors ................................................... ix 1 Cell Senescence as Both a Dynamic and a Static Phenotype ..... 1 Andrew R.J. Young, Masako Narita, and Masashi Narita 2 Senescence Regulation by mTOR ............................... 15 Vjekoslav Dulic 3 Senescence Regulation by the p53 Protein Family ............. 37 Yingjuan Qian and Xinbin Chen 4 Markers of Cellular Senescence .............................. 63 Amancio Carnero 5 Biomarkers of Cell Senescence Assessed by Imaging Cytometry ................................................... 83 Hong Zhao and Zbigniew Darzynkiewicz 6 Cytofluorometric Assessment of Cell Cycle Progression ....... 93 Ilio Vitale, Mohamed Jemaà, Lorenzo Galluzzi, Didier Metivier, Maria Castedo, and Guido Kroemer 7 Quantification of Cell Cycle-Arresting Proteins ............ 121 Oliver Kepp, Isabelle Martins, Laurie Menger, Mickael Michaud, Sandy Adjemian, Abdul Qader Sukkurwala, Lorenzo Galluzzi, and Guido Kroemer 8 Colorimetric Detection of Senescence-Associated (3 Galactosidase ........................................... 143 Koji Itahana, Yoko Itahana, and Goberdhan P. Dimri 9 Chemiluminescent Detection of Senescence-Associated p Galactosidase .............................................. 157 Vinicius Bassaneze, Ayumi Aurea Miyakawa, and José Eduardo Krieger 10 Detection of the Senescence-Associated Secretory Phenotype (SASP) ........................................... 165 Francis Rodier 11 Unbiased Characterization of the Senescence-Associated Secretome Using SILAC-Based Quantitative Proteomics ........ 175 Juan Carlos Acosta, Ambrosius P. Snijders, and Jesús Gil 12 Detection of Senescence-Associated Heterochromatin Foci (SAHF) ..................................................... 185 Katherine M. Aird and Rugang Zhang 13 Monitoring DNA Damage During Cell Senescence ............... 197 Glyn Nelson and Thomas von Zglinicki 14 Assessment and Quantification of Telomerase Enzyme Activity ................................................... 215 Michelle F. Maritz, Laura A. Richards, and Karen L. MacKenzie 15 Methods for the Assessment of Telomere Status .............. 233 Asako J. Nakamura 16 Detection of Nuclear Envelope Alterations in Senescence .... 243 Clea Bárcena, Fernando G. Osorio, and Jose Maria Pérez Freije 17 Measuring Reactive Oxygen Species in Senescent Cells ....... 253 João F. Passes, Satomi Miwa, and Thomas von Zglinicki 18 Quantification of Protein Carbonylation .................... 265 Nancy B. Wehr and Rodney L. Levine 19 Assays for the Measurement of Lipid Peroxidation ........... 283 Ana Cipak Gasparovic, Morana Jaganjac, Branka Mihaljevic, Suzana Borovic Sunjic, and Neven Zarkovic 20 Raman Spectroscopy for the Detection of AGEs/ALEs .......... 297 J. Renwick Beattie, John J. McGarvey, and Alan W. Stitt 21 Monitoring Oncogenic B-RAF-Induced Senescence in Melanocytes ................................................ 313 Sieu L. Tran and Helen Rizos 22 Methods to Investigate the Role of SIRT1 in Endothelial Senescence ................................................. 327 Bo Bai and Yu Wang 23 Monitoring Nutrient Signaling Through the Longevity Protein p66SHCI ............................................ 341 Sofia Chiatamone Ranieri and Giovambattista Pani 24 Profiling the Metabolic Signature of Senescence ............ 355 Florian M. Geier, Silke Fuchs, Gabriel Valbuena, Armand M. Leroi, and Jacob G. Bundy 25 Genome-Wide RNAi Screening to Identify Regulators of Oncogene-Induced Cellular Senescence ....................... 373 Narendra Wajapeyee, Sara K. Deibler, and Michael R. Green 26 An Integrated Approach for Monitoring Cell Senescence ...... 383 Tatiana V. Pospelova, Zhanna V. Chitikova, and Valery A. Pospelov 27 Robust Multiparametric Assessment of Cellular Senescence ... 409 Clara Correia-Melo, Diana Jurk, and João F. Passes 28 Assessing Chronological Aging in Bacteria .................. 421 Stavros Gonidakis and Valter D. Longo 29 Assessing Organismal Aging in the Filamentous Fungus Podospora anserina ......................................... 439 Heinz D. Osiewacz, Andrea Hamann, and Sandra Zintel 30 Assessing Chronological Aging in Saccharomyces cerevisiae .. 463 Jia Нu, Min Wei, Mario G. Mirisola, and Valter D. Longo 31 Assessing Aging and Senescent Decline in Caenorhabditis elegans: Cohort Survival Analysis .......................... 473 Eirini Lionaki and Nektarios Tavernarakis 32 High-Throughput and Longitudinal Analysis of Aging and Senescent Decline in Caenorhabditis elegans ................ 485 Eirini Lionaki and Nektarios Tavernarakis 33 Assessing Senescence in Drosophila Using Video Tracking .... 501 Reza Ardekani, Simon Tavaré, and John Tower 34 Assessing Vascular Senescence in Zebrafish ................. 517 Sandra Donnini, Antonio Giachetti, and Marina Ziche Index ......................................................... 533