Antibody engineering: methods and protocols / ed. by P.Chames. - 2nd ed. - New York: Humana Press/Springer, 2012. - xvi, 734 p.: ill. - (Methods in molecular biology; 907) (Springer protocols). - Incl. bibl. ref. - Ind.: p.729-734. - ISBN 978-1-61779-973-0; ISSN 1064-3745  Место хранения:   056 | Институт химической биологии и фундаментальной медицины CO РАН | Новосибирск| Библиотека

Оглавление / Contents

Preface ......................................................... v Contributors ................................................... xi Part I In Silico Methods 1 Use of IMGT® Databases and Tools for Antibody Engineering and Humanization ............................................. 3 Marie-Paule Lefranc, Francois Ehrenmann, Chantal Ginestoux, Veronique Giudicelli, and Patrice Duroux 2 Computer-Assisted Modeling of Antibody Variable Domains ..... 39 Oscar H.P. Ramos Part II Generation of Diversity 3 Cloning Single-Chain Antibody Fragments (ScFv) from Hyrbidoma Cells ............................................. 59 Lars Toleikis and Andre Frenzel 4 Human In-Cell scFv Library from Infiltrating В Cell ......... 73 Sylvie Peraldi-Roux 5 Construction of Human Naive Antibody Gene Libraries ......... 85 Michael Hust, André Frenzel, Torsten Meyer, Thomas Schirrmann, and Stefan Dübel 6 Synthetic Customized scFv Libraries ........................ 109 Gautier Robin and Pierre Martineau 7 Selection of Stable scFv Antibodies by Phage Display ....... 123 Eeva-Christine Brockmann 8 Generation of Single Domain Antibody Fragments Derived from Camelids and Generation of Manifold Constructs ........ 145 Cécile Vincke, Carlos Gutiérrez, Ulrich Wernery, Nick Devoogdt, Gholamreza Hassanzadeh-Ghassabeh, and Serge Muyldermans 9 Generation and Isolation of Target-Specific Single-Domain Antibodies from Shark Immune Repertoires ................... 177 Mischa Roland Müller, Ronan O'Dwyer, Marina Kovaleva, Fiona Rudkin, Helen Dooley, and Caroline Jane Barelle 10 Generation of Human Single Domain Antibody Repertoires by Kunkel Mutagenesis ...................................... 195 Remain Rouet, Kip Dudgeon, and Daniel Christ Part III Selections of Lead Candidate 11 Phage Display and Selections on Purified Antigens .......... 213 Julie Matz and Patrick Chames 12 Phage Display and Selections on Cells ...................... 225 Klervi Even-Desrumeaux and Patrick Chames 13 Humanization by CDR Grafting and Specificity-Determining Residue Grafting ........................................... 237 Jin Hong Kim and Hyo Jeong Hong 14 Humanization by Guided Selections .......................... 247 Sang Jick Kim and Hyo Jeong Hong 15 Selection of Antibody Fragments by Yeast Display ........... 259 Nathalie Scholler 16 Evolution of Antibodies In Vitro by Ribosome Display ....... 281 Bryan M. Edwards and Mingyue He 17 Mammalian Cell Surface Display of Full Length IgG .......... 293 Chen Zhou and Wenyan David Shen Part IV Production of Recombinant Antibodies 18 Production of Antibody Fragments in Escherichia coli ....... 305 Tomohisa Katsuda, Hiroyuki Sonoda, Yoichi Kumada, and Hideki Yamaji 19 Production of Antibody Derivatives in the Methylotrophic Yeast Pichia pastoris ...................................... 325 Steve Schoonooghe, Jannick Leoen, and Jurgen Haustraete 20 Monoclonal Antibody Expression in Mammalian Cells .......... 341 Richard Yi Zhang and Wenyan David Shen 21 Production of Recombinant Antibodies in Drosophila melanogaster S2 Cells ...................................... 359 Daniel X. Johansson, Thomas Krey, and Oskar Andersson 22 Production of Antibody Fragments Using the Baculovirus- Insect Cell System ......................................... 371 Takanori Furuta, Takafumi Ogawa, and Hideki Yamaji 23 Transient and Stable Expression of Antibodies in Nicotiana Species .......................................... 389 Freydoun Garabagi, Michael D. McLean, and J. Christopher Hall Part V Variable Domain Optimization 24 Measuring Antibody-Antigen Binding Kinetics Using Surface Plasmon Resonance .......................................... 411 Stephen Hearty, Paul Leonard, and Richard O'Kennedy 25 Affinity Determination of Biotinylated Antibodies by Flow Cytometry .................................................. 443 Klervi Even-Desrumeaux and Patrick Chames 26 Affinity Maturation of Antibodies: Optimized Methods to Generate High-Quality ScFv Libraries and Isolate IgG Candidates by High-Throughput Screening .................... 451 Laurence Renaut, Céline Monnet, Olivier Dubreuil, Ouafa Zaki, Fabien Crozet, Khalil Bouayadi, Hakim Kharrat, and Philippe Mondon 27 Affinity Maturation by Semi-rational Approaches ............ 463 Rodrigo Barderas, Johan Desmet, Philippe Alard, and J. Ignacio Casal 28 Molecular Scanning: Combining Random Mutagenesis, Ribosome Display, and Bioinformatic Analysis for Protein Engineering ................................................ 487 Alfredo Darmanin-Sheehan, William James Jonathan Finlay, Orla Cunningham, and Brian Joseph Fennell Part VI Fc Engineering 29 Fucose-Targeted Glycoengineering of Pharmaceutical Cell Lines ...................................................... 507 Christiane Ogorek, Ingo Jordan, Volker Sandig, and Hans Henning von Horsten 30 Fc Engineering: Design, Expression, and Functional Characterization of Antibody Variants with Improved Effector Function .......................................... 519 Stefanie Derer, Christian Kellner, Sven Berger, Thomas Valerius, and Matthias Peipp 31 Fc Engineering: Serum Half-Life Modulation Through FcRn Binding .................................................... 537 Tove Olaften 32 Monoclonal Antibody Lead Characterization: In Vitro and In Vivo Methods ............................................ 557 Axel Hernandez, Julie Parmentier, Touzhen Wang, Jane Cheng, and Gadi Gazit Bornstein Part VII Innovative Formats 33 Production, Purification, and Characterization of scFv TNF Ligand Fusion Proteins ................................. 597 Andrea Pick, Agnes Wyzgol, and Harald Wajant 34 Antibody-IL2 Fusion Proteins for Tumor Targeting ........... 611 Andreas A. Hombach and Hinrich Abken 35 Recombinant Immunotoxins with Low Endotoxins for Clinical and Animal Studies ......................................... 627 Masanori Onda 36 Chimeric Antigen Receptors for T-Cell Based Therapy ........ 645 Eleanor J. Cheadle, Vicky Sheard, Andreas A. Hombach, Markus Chmielewski, Tobias Riet, Cor Berrevoets, Erik Schooten, Cor Lamers, Hinrich Abken, Reno Debets, and David E. Gilham 37 Selection and Use of Intracellular Antibodies (Intrabodies) .............................................. 667 Sandrine Moutel, Clément Nizak, and Franck Perez 38 Radiolabeled Antibodies for Cancer Imaging and Therapy ..... 681 Jacques Barbet, Manuel Bardiès, Mickael Bourgeois, Jean-François Chatal, Michel Chérel, François Davodeau, Alain Faivre-Chauvet, Jean-François Gestin, and Frangoise Kraeber-Bodéré 39 Design and Production of Multimeric Antibody Fragments, Focused on Diabodies with Enhanced Clinical Efficacy ....... 699 Glenn A. Powers, Peter J. Hudson, and Michael P. Wheatcroft 40 Production of Bispecific Antibodies: Diabodies and Tandem scFv ....................................................... 713 Nora Hornig and Aline Färber-Schwarz Index ......................................................... 729