Preface ......................................................... v
Contributors ................................................... ix
1 Therapeutic Oligonucleotides ................................. 1
John Goodchild
2 Dinucleotides Containing 3'-5-Phosphorothiolate Linkages .... 17
James W. Gaynor and Richard Cosstick
3 2'-O,4'-C-Methyleneoxymethylene Bridged Nucleic Acids
(2',4'-BNAcoc) .............................................. 31
Yoshiyuki Hari, Tetsuya Kodama, Takeshi Imanishi,
and Satoshi Obika
4 A Non-covalent Peptide-Based Strategy for Ex Vivo and In
Vivo Oligonucleotide Delivery ............................... 59
Laurence Crombez, May C. Morris, Frederic Heitz, and
Gilles Divita
5 Cell-Penetrating Peptides-Based Strategies for the
Delivery of Splice Redirecting Antisense Oligonucleotides ... 75
Samir El Andaloussi, Fatouma Said Hassane, Prisca
Boisguerin, Rannar Sillard, Ülo Langel, and Bernard Lebleu
6 A Nanoparticle for Tumor Targeted Delivery of Oligomers ..... 91
Xinrong Liu, Yi Wang, and Donald J. Hnatowich
7 Light-Directed Delivery of Nucleic Acids ................... 107
Sigurd Bøe, Lina Prasmickaite, Birgit Engesater, and
Eivind Hovig
8 Antibody Targeted siRNA Delivery ........................... 123
Masoud M. Toloue and Lance P. Ford
9 Aptamer-Drug Conjugation for Targeted Tumor Cell Therapy ... 141
Michael J. Donovan, Ling Meng, Too Chen, Tunfei Zhang,
Kwame Sefah, and Weihong Tan
10 Five-Step Process for Screening Antisense Compounds for
Efficacy: Gene Target IL-12Rb2 ............................. 153
Nikki B. Marshall, Laura L. Hauck, and Dan V. Meurich
11 Diverse Small Non-coding RNAs in RNA Interference
Pathways ................................................... 169
Liande Li and Yi Liu
12 Quantification of si RNAs In Vitro and In Vivo ............. 183
Angie Cheng, Alexander V. Vlassov, and Susan Magdaleno
13 Optimization of Transfection Conditions and Analysis of
siRNA Potency Using Real-time PCR .......................... 199
Angle Cheng, Susan Magdalena, and Alexander V. Vlassov
14 siRNA Knockdown of Gene Expression in Endothelial Cells .... 215
Emily Dennstedt and Brad Bryan
15 Using RNA Interference in Schistosoma mansoni .............. 223
Rita Bhardwaj, Greice Krautz-Peterson, and Patrick
J. Skelly
16 Performing the Labeled microRNA Pull-Down (LAMP) Assay
System: An Experimental Approach for High-Throughput
Identification of microRNA-Target mRNAs .................... 241
Ren-Jun Hsu and Huai-Jen Tsai
17 Synthesis, Purification, and Characterization of
Oligoribonucleotides that Act as Agonists of TLR7 and/or
TLR8 ....................................................... 249
Tao Lan and Ekambar R. Kandimalla
18 Synthesis, Purification, and Characterization of Immune-
Modulatory Oligodeoxynucleotides that Act as Agonists of
Toll-Like Receptor 9 ....................................... 263
Mallikarjuna Reddy Putta, Dong Yu, and Ekambar R.
Kandimalla
19 Surface Plasmon Resonance Investigation of RNA Aptamer-
RNA Ligand Interactions .................................... 279
Carmelo Di Primo, Eric Dausse, and Jean-Jacques Toulmé
20 Practical Considerations for Analyzing Antigene RNAs
(agRNAs): RNA Immunoprecipitation of Argonaute Protein ..... 301
Jacob C. Schwartz and David R. Corey
21 Inhibition of Human Papillomavirus Expression Using
DNAzymes ................................................... 317
Maria Luisa Benitez-Hess, Pablo Reyes-Gutiérrez,
and Luis Marat Alvarez-Salas
Index ......................................................... 337
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