Preface ......................................................... v
Contributors ................................................... ix
1 Historical Overview of Nanotechnology and Nanotoxicology ..... 1
Annette Santamaria
2 Characterization of Nanomaterials for Toxicological
Studies ..................................................... 13
Kevin W. Powers, Paul L. Carpinone, and Kerry N. Siebein
3 Methods for Understanding the Interaction Between
Nanoparticles and Cells ..................................... 33
Pilar Rivera Gil, Martin J.D. Clift, Barbara Rothen
Rutishauser, and Wolfgang J. Parak
4 Single-Cell Gel Electrophoresis (Comet) Assay in Nano-
genotoxicology .............................................. 57
Maricica Pacurari and Vincent Castranova
5 Single-Cell Nanotoxicity Assays of Superparamagnetic Iron
Oxide Nanoparticles ......................................... 69
Trisha Eustaquio and James F. Leary
6 Western Blot Analysis ....................................... 87
Seishiro Hirano
7 Application of Reverse Transcription-PCR and Real-Time
PCR in Nanotoxicity Research ................................ 99
Yiqun Mo, Rong Wan, and Qunrvei Zhang
8 Deriving TC50 Values of Nanoparticles from Electrochemical
Monitoring of Lactate Dehydrogenase Activity Indirectly .... 113
Fuping Zhang, Na Wang, Fang Chang, and Shuping Bi
9 Enzyme-Linked Immunosorbent Assay of IL-8 Production
in Response to Silver Nanoparticles ........................ 131
Eun-Jeong Yang, Jiyoung Jang, Dae-Hyoun Lim, and In-Hong
Choi
10 Metabolomics Techniques in Nanotoxicology Studies .......... 141
Laura K. Schnackenberg, Jinchun Sun, and Richard D. Beger
11 Nanoparticle Uptake Measured by Flow Cytometry ............. 157
Yuko Ibuki and Tatsushi Toyooka
12 Determining Biological Activity of Nanoparticles as
Measured by Flow Cytometry ................................. 167
Jennifer F. Nyland
13 Whole Cell Impedance Biosensoring Devices .................. 177
Evangelia Hondroulis and Chen-Zhong Li
14 Free Energy Calculation of Permeant-Membrane Interactions
Using Molecular Dynamics Simulations ....................... 189
Paolo Elvati and Angela Violi
15 Screening of Fullerene Toxicity by Hemolysis Assay ......... 203
Federica Tramer, Tatiana Da Ros, and Sabina Passamonti
16 Assessment of In Vitro Skin Irritation Potential
of Nanoparticles: RHE Model ................................ 219
P. Balakrishna Murthy, A. Sairam Kishore, and P. Surekha
17 In Vivo Methods of Nanotoxicology .......................... 235
Khaled Greish, Giridhar Thiagarajan, and Hamidreza
Ghandehari
18 The Luminescent Bacteria Test to Determine the Acute
Toxicity of Nanoparticle Suspensions ....................... 255
Ana Garcia, Sonia Recillas, Antoni Sánchez, and Xavier
Font
19 The Primacy of Physicochemical Characterization of
Nanomaterials for Reliable Toxicity Assessment: A Review
of the Zebrafish Nanotoxicology Model ...................... 261
John P. Bohnsack, Shoeleh Assemi, Jan D. Miller, and
Darin Y. Furgeson
20 Application of Embryonic and Adult Zebrafish for
Nanotoxicity Assessment .................................... 317
Jiangxin Wang, Xiaoshan Zhu, Yongsheng Chen, and Yung
Chang
21 Applications of Subsurface Microscopy ...................... 331
Laurene Tetard, Ali Passian, Rubye H. Farahi, Brynn H.
Voy, and Thomas Thundat
22 Application of ICP-MS for the Study of Disposition and
Toxicity of Metal-Based Nanomaterials ...................... 345
Mo-Hsiung Yang, Chia-Hua Lin, Louis W. Chang, and Pinpin
Lin
23 Quantitative Nanoparticle Organ Disposition by Gel
Permeation Chromatography .................................. 361
Abdul Khader Mohammad and Joshua Reineke
24 Physiologically Based Pharmacokinetic Modeling for
Nanoparticle Toxicity Study ................................ 369
Mingguang Li and Joshua Reineke
25 Biophysical Methods for Assessing Plant Responses to
Nanoparticle Exposure ...................................... 383
Tatsiana A. Ratnikova, Ran Chen, Priyanka Bhattacharya,
and Pu Chun Ke
26 In Vivo Nanotoxicity Assays in Plant Models ................ 399
Mamta Kumari, Vinita Ernest, Amitava Mukherjee, and
Natarajan Chandrasekaran
Index ......................................................... 411
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