Extracellular matrix protocols / ed. by S.Even-Ram, V.V.Artym. - 2nd ed. - New York: Humana, 2009. - xi, 375 p.: ill. - (Methods in molecular biology; 522) (Springer protocols). - Incl. bibl. ref. - Ind.: p.371-375. - ISBN 978-1-58829-984-0; ISSN 1064-3745  Место хранения:   056 | Институт химической биологии и фундаментальной медицины CO РАН | Новосибирск| Библиотека

Оглавление / Contents

Preface ......................................................... v Contributors ................................................... ix Part I: Molecular Biology 1 Retroviral Delivery of ECM Genes ............................. 3 Vitali Alexeev and Olga Igoucheva 2 Tissue-Specific КО of ECM Proteins .......................... 15 Mara Brancaccio, Emila Turco, and Emilio Hirsch 3 Recombinant Collagen Trimers from Insect Cells and Yeast .... 51 Johanna Myllyharju 4 Eukaryotic Expression and Purification of Recombinant Extracellular Matrix Proteins Carrying the Strep II Tag ..... 63 Neil Smyth, Uwe Odenthal, Barbara Merkl, and Mats Paulsson 5 Preparation of Recombinant Fibronectin Fragments for Functional and Structural Studies ........................... 73 David Staunton, Christopher J. Millard, A. Radu Aricescu, and Iain D. Campbell Part II: Biochemical and Biophysical Analysis 6 Quantitative Determination of Collagen Cross-links ......... 103 Nicholas C. Avery, Trevor J. Sims, and Allen J. Bailey 7 ECM Macromolecules: Height-Mapping and Nano-Mechanics Using Atomic Force Microscopy .............................. 123 Nigel W. Hodson, Cay M. Kielty, and Michael J. Sherratt 8 Atomic Force Microscopy Measurements of Intermolecular Binding Forces ............................................. 143 Gradimir N. Misevic, Yannis Karamanos, and Nikola J. Misevic 9 Mass-Mapping of ECM Macromolecules by Scanning Transmission Electron Microscopy ............................ 151 Michael J. Sherratt, Helen K. Graham, Cay M. Kielty, and David F. Holmes 10 Chemical Microscopy of Biological Samples by Dynamic Mode Secondary Ion Mass Spectrometry ............................. 163 Gradimir N. Misevic, Bernard Rasser, Vic Norris, Cédric Dérue, David Gibouin, Fabrice Lefebvre, Marie-Claire Verdus, Anthony Delaune,Guillaume Legent, and Camille Ripoll 11 ECM Macromolecules: Rotary Shadowing and Transmission Electron Microscopy ......................................... 175 Michael J. Sherratt, Roger S. Meadows, Helen K. Graham, Cay M. Kielty, and David F. Holmes 12 Using Self-Assembled Monolayers to Pattern ECM Proteins and Cells on Substrates ..................................... 183 Emanuele Ostuni, George M. Whitesides, Donald E. Ingber, and Christopher S. Chen 13 Solid Phase Assays for Studying ECM Protein-Protein Interactions ................................................ 195 A Paul Mould Part III: Cell Biology Assays 14 Cell Adhesion Assays ........................................ 203 Martin J. Humphries 15 ECM Degradation Assays for Analyzing Local Cell Invasion .... 211 Vira V. Artym, Kenneth M. Yamada, and Susette C. Mueller 16 Fluorescence-Based Assays for In Vitro Analysis of Cell Adhesion and Migration ...................................... 221 Paola Spessotto, Katia Lacrima, Pier Andrea Nicolosi, Eliana Pivetta, Martina Scapolan, and Roberto Perris 17 Fibrin Gel Model for Assessment of Cellular Contractility ... 251 Sharona Even-Ram 18 Fluorescent Labeling Techniques for Investigation of Fibronectin Fibrillogenesis (Labeling Fibronectin Fibrillogenesis) ............................................ 261 Roumen Pankov and Albena Momchilova 19 Stromagenesis During Tumorigenesis: Characterization of Tumor-Associated Fibroblasts and Stroma-Derived 3D Matrices .................................................... 275 Remedies Castelló-Cros and Edna Cukierman Part IV: Organ Models 20 Tissue Recombinants to Study Extracellular Matrix Targeting to Basement Membranes ............................ 309 Patricia Simon-Assmann, Anne-Laure Bolcato-Bellemin, Annick Klein, and Michèle Kedinger 21 ECM and FGF-Dependent Assay of Embryonic SMG Epithelial Morphogenesis: Investigating Growth Factor/Matrix Regulation of Gene Expression During Submandibular Gland Development ................................................ 319 Ivan T. Rebustini and Matthew P. Hoffman 22 Analyzing how Cell Adhesion Controls Mammary Gland Function by Transplantation of Embryonic Mammary Tissue from Knockout Mice ......................................... 331 Teresa C.M. Klinowska and Charles H. Streuli Part V: Tissue Engineering 23 Characterizing ECM Production by Cells Encapsulated in Hydrogels .................................................. 349 Iossif A. Strehin and Jennifer H. Elisseeff 24 Issue Engineering and Cell-Populated Collagen Matrices ..... 363 Paul D. Kemp Index ......................................................... 371