Preface ....................................................... vii
Foreword ....................................................... ix
Contributors ................................................... xv
1 From Neanderthal to Nanobiotech: From Plant Potions to
Pharming with Plant Factories ................................ 1
Christophe Sourrouille, Brian Marshall, David Liénard, and
Loїc Faye
2 Cowpea Mosaic Virus-Based Systems for the Expression
of Antigens and Antibodies in Plants ........................ 25
Frank Sainsbury, Li Liu, and George P. Lomonossoff
3 Transient Expression of Antibodies in Plants Using Syringe
Agroinfiltration ............................................ 41
Marc-Andre D'Aoust, Pierre-Olivier Lavoie, Julie Belles-
Isles, Nicole Bechtold, Michèle Martel, and Louis-P.
Vézina
4 Rapid System for Evaluating Bioproduction Capacity of
Complex Pharmaceutical Proteins in Plants ................... 51
Giuliana Medrano, Michael J. Reidy, Jianyun Liu, Jorge
Ayala, Maureen C. Dolan, and Carole L. Cramer
5 Production and Localization of Recombinant Pharmaceuticals
in Transgenic Seeds ......................................... 69
Thomas Rademacher, Elsa Arcalis, and Eva Stoger
6 Production of Antibody Fragments in Arabidopsis Seeds ....... 89
Bart Van Droogenbroeck, Kirsten De Wilde, and Ann Depicker
7 Production of Plantibodies in Nicotiana Plants ............. 103
Marta Ayala, Jorge Gavilondo, Meilyn Rodríguez, Alejandro
Fuentes, Gil Enríquez, Lincidio Pérez, José Cremata, and
Merardo Pujol
8 Physcomitrella Patens: A Non-Vascular Plant for
Recombinant Protein Production ............................. 135
David Liénard and Fabien Nogué
9 Production of Recombinant Proteins in Suspension-Cultured
Plant Cells ................................................ 145
Carole Plasson, Rémy Michel, David Lienard, Claude Saint-
Jore-Dupas, Christophe Sourrouille, Ghislaine Grenier de
March, and Véronique Gomord
10 Chloroplast-Derived Vaccine Antigens and
Biopharmaceuticals: Protocols for Expression,
Purification, or Oral Delivery and Functional Evaluation ... 163
N. Dolendro Singh, Yi Ding, and Henry Daniell
11 Protein Body Induction: A New Tool to Produce and Recover
Recombinant Proteins in Plants ............................. 193
Margarita Torrent, Imma Llop-Tous, and M. Dolors Ludevid
12 A Case Study for Plant-Made Pharmaceuticals Comparing
Different Plant Expression and Production Systems .......... 209
Guy Vancanneyt, Manuel Dubald, Werner Schröder, Jörg
Peters, and Johan Botterman
13 Glycosylation of Antibody Therapeutics: Optimisation for
Purpose .................................................... 223
Roy Jefferis
14 N-Glycosylation of Plant Recombinant Pharmaceuticals ....... 239
Muriel Bardor, Gleysin Cabrera, Johannes Stadlmann,
Patrice Lerouge, José A. Cremata, Véronique Gomord and
Anne-Catherine Fitchette
15 Companion Protease Inhibitors to Protect Recombinant
Proteins in Transgenic Plant Extracts ...................... 265
Meriem Benchabane, Daniel Rivard, Cécile Girard, and
Dominique Michaud
16 Strategies for Improving Vaccine Antigens Expression in
Transgenic Plants: Fusion to Carrier Sequences ............. 275
Jose M. Escribano and Daniel M. Perez-Filgueira
17 Immunomodulation of Plant Function by In Vitro Selected
Single-Chain Fv Intrabodies ................................ 289
Manfred Gahrtz and Udo Conrad
18 On-Chip Detection of Low-Molecular-Weight Recombinant
Proteins in Plant Crude Extracts by SELDI-TOF MS ........... 313
Amine M. Badri, Karine Coenen, Louis-Philippe
Vaillancourt, Charles Goulet, and Dominique Michaud
19 Assessing the Risk of Undesirable Immunogenicity/
Allergenicity of Plant-Derived Therapeutic Proteins ........ 325
Paul D. Chamberlain
20 Biosafety, Risk Assessment and Regulation of Plant-Made
Pharmaceuticals ............................................ 341
Penelope A.C. Sparrow and Richard M. Twyman
Index ......................................................... 355
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