Preface ......................................................... v
Contributors ................................................... ix
1 Multiple Dimensions in Plant Signal Transduction: An
Overview ..................................................... 1
Jorge J. Casal
2 Phenotyping of Arabidopsis Mutants for Developmental
Effects of Gene Deletions ................................... 17
Cordelia Bolle
3 Phenotyping of Abiotic Responses and Hormone Treatments
in Arabidopsis .............................................. 35
Cordelia Bolle
4 Accurate Real-time Reverse Transcription Quantitative PCR ... 61
Marco Klatte and Petra Bauer
5 Probing Spatio-temporal Intracellular Calcium Variations
in Plants ................................................... 79
Axel Mithöfer, Christian Mazars, and Massimo E. Maffei
6 Dynamic Redox Measurements with Redox-Sensitive GFP in
Plants by Confocal Laser Scanning Microscopy ................ 93
Andreas J. Meyer and Thorsten Brach
7 Imaging of Reactive Oxygen Species in vivo ................. 109
Steven M. Driever, Michael J. Fryer, Philip M. Mullineaux,
and Neil R. Baker
8 Identification of Thioredoxin Targeted Proteins Using
Thioredoxin Single Cysteine Mutant-immobilized Resin ....... 117
Ken Motohashi, Patrick G.N. Romano, and Toru Hisabori
9 Determination of in vivo Protein Phosphorylation in
Photosynthetic Membranes ................................... 133
Julia P. Vainonen, Alexander V. Vener, and Eva-Mari Aro
10 Examining Protein Stability and Its Relevance for Plant
Growth and Development ..................................... 147
Claus Schwechheimer, Björn C. Willige, Melina Zourelidou,
and Esther M.N. Dohmann
11 Probing Circadian Rhythms in Chlamydomonas rheinhardtii
by Functional Proteomics ................................... 173
Volker Wagner and Maria Mittag
12 Bimolecular Fluorescence Complementation (BiFC) to Study
Protein-protein Interactions in Living Plant Cells ......... 189
Katia Schütze, Klaus Harter, and Christina Chaban
13 Fluorescence Cross-Correlation Spectroscopy of Plant
Proteins ................................................... 203
Hideki Muto, Masataka Kinjo, and Kotaro T. Yamamoto
14 The Determination of Protein-protein Interactions by the
Mating-based Split-ubiquitin-system (mbSUS) ................ 217
Christopher Grefen, Petr Obrdlik, and Klaus Harter
15 Functional Complementation of Yeast Mutants to Study
Plant Signalling Pathways .................................. 235
Norbert Mehlmer, Elisabeth Scheikl-Pourkhalil, and Markus
Teige
16 Phosphatase Activities Analyzed by in vivo Expressions ..... 247
Alois Schweighofer, Zahra Ayatollahi, and Irute Meskiene
17 Chromatin Immunoprecipitation Experiments to Investigate
in vivo Binding of Arabidopsis Transcription Factors to
Target Sequences ........................................... 261
Benjamin Fode and Christiane Gatz
18 Fluorescence-based Electrophoretic Mobility Shift Assay
in the Analysis of DNA-binding Proteins .................... 273
Sebastian Steiner and Thomas Pfannschmidt
19 Analysis of Plant Regulatory DNA sequences by the Yeast-
One-Hybrid Assay ........................................... 291
Dierk Wanke and Klaus Harter
20 Analysis of Plant Regulatory DNA Sequences by Transient
Protoplast Assays and Computer Aided Sequence Evaluation ... 311
Kenneth W. Berendzen, Klaus Harter, and Dierk Wanke
21 RNA-protein Interaction Mediating Post-transcriptional
Regulation in the Circadian System ......................... 337
Jan C. Schöning and Dorothee Staiger
Index ......................................................... 353
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