Molecular beacons: signalling nucleic acid probes, methods, and protocols (Totowa, 2008). - ОГЛАВЛЕНИЕ / CONTENTS
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ОбложкаMolecular beacons: signalling nucleic acid probes, methods, and protocols / ed. by A.Marx, O.Seitz. - Totowa: Humana Press, 2008. - xii, 266 p.: ill. (some col.). - (Methods in molecular biology; 429). - Incl. bibl. ref. - Ind.: p.265-266. - ISBN 978-1-58829-700-6
 

Оглавление / Contents
 
   Preface ...................................................... v
   Contributors ................................................ xi

I  Introduction

1  Basic Principles of Fluorescence and Energy Transfer ......... 3
   Larry E. Morrison

II  Applications

2  Multiplex Detection of Mutations ............................ 23
   David S. Perlin, Sergey Balashov, and Steven Park
3  Detection of Salmonella Species in Foodstuffs ............... 33
   Arvind A. Bhagwat, Jitu Patel, Trina Chua, Audrey Chan,
   Saúl Ruiz Cruz, and Gustavo A. González Aguilar
4  Identification of Homozygous Transgenic Mice by Genomic 
   Real-Time PCR ............................................... 45
   Gunter Schmidtke and Marcus Groettrup
5  Quantitative Analysis of Gene Expression Relative to 18S
   rRNA in Carcinoma Samples Using the LightCycler® 
   Instrument and a SYBR Greenl-based Assay: Determining 
   FAT10 mRNA Levels in Hepatocellular Carcinoma ............... 59
   Sebastian Lukasiak, Kai Breuhahn, Claudia Schiller, Gunter
   Schmidtke, and Marcus Groettrup
6  Absolute Quantitation of Feline Leukemia Virus Proviral
   DNA and Viral RNA Loads by TaqMan® Real-Time PCR and
   RT-PCR ...................................................... 73
   Valentino Cattori and Regina Hofmann-Lehmann
7  High-Throughput Real-Time PCR ............................... 89
   Thomas D. Schmittgen, Eun Joo Lee, and Jinmai Jiang
8  Design and Use of Scorpions Fluorescent Signalling
   Molecules ................................................... 99
   Rachael Carters, Jennifer Ferguson, Rupert Gaut, Paul 
   Ravetto, Nicola Thelwell, and David Whitcombe
9  Hybridization Probe Pairs and Single-Labeled Probes: an
   Alternative Approach for Genotyping and Quantification ..... 117
   Thomas Froehlich and Oliver Geulen

III New Technologies

10 EasyBeaconsTM for the Detection of Methylation Status 
   of Single CpG Duplets ...................................... 137
   Ulf B. Christensen
11 Quenched Autoligation Probes ............................... 161
   Adam P. Silverman, Hiroshi Abe, and Eric T. Kool
12 HyBeacon® Probes for Rapid DNA Sequence Detection and 
   Allele Discrimination ...................................... 171
   David J. French, David G. McDowell, Paul Debenham, 
   Nittaya Gale, and Tom Brown
13 FIT-Probes in Real-Time PCR ................................ 187
   Elke Socher and Oliver Seitz
14 SNP Genotyping by Unlabeled Probe Melting Analysis ......... 199
   Maria Erali, Robert Palais, and Carl Wittwer

IV  Non-Nucleic Acid Targets

15 Molecular Beacons for Protein —DNA Interaction Studies ..... 209
   Jun Li, Zehui Charles Cao, Zhiwen Tang, Kemin Wang, and
   Weihong Tan
16 DNA Polymerase Profiling ................................... 225
   D. Summerer
17 Modular Reporter Hairpin Ribozymes for Analyzing 
   Molecular Interactions ..................................... 237
   S. Hani Najafi-Shoushtari and Michael Famulok
18 Screening of Molecular Interactions Using Reporter
   Hammerhead Ribozymes ....................................... 251
   Jörg S. Hartig and Michael Famulok

   Index ...................................................... 265


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