Preface. By Otto S. Wolfbeis ................................... xi
Part I. Fluorescence in High-Throughput Screening
Single-bead, Single-molecule, Single-cell Fluorescence:
Technologies for Drug Screening and Target Validation.
By Martin Hintersteiner and Manfred Auer ..................... 1
Substrate Arrays for Fluorescence-Based Enzyme
Fingerprinting and High-Throughput Screening.
By Jean-Louis Reymond ....................................... 12
Part II. Fluorescence Spectroscopy
Probing the Interior of Living Cells with
Fluorescence Correlation Spectroscopy.
By Matthias Weiss ........................................... 21
Fluorescence Measurements on Functionalized Polymer
Surfaces—Problems and Troubleshooting.
By Katrin Hoffmann, Renate Mix, Ute Resch-Genger,
and Joerg F. Friedrich ...................................... 28
Standardization of Fluorescence Measurements: Criteria
for the Choice of Suitable Standards and Approaches to
Fit-for-Purpose Calibration Tools.
By Ute Resch-Genger, Katrin Hoffmann, and
Angelika Hoffmann ........................................... 35
Investigating Transcriptional Regulation by Fluorescence
Spectroscopy, from Traditional Methods to State-of-the-Art
Single-Molecule Approaches.
By Silvia Zorrilla, M. Pilar Lillo, Denis Chaix, Emmanuel
Margeat, Catherine A. Royer, and Nathalie Declerck .......... 44
Subpicosecond Transient Signal Spectroscopy of Prodan in
Dimethylformamide Solution.
By János Erostyák, Pasi Myllyperkiö,
Andrea Buzády, and Jouko Korppi-Tommola ..................... 52
Frequency Domain Fluorometry with Pulsed
Light-Emitting Diodes.
By Petr Herman and Jaroslav Vecer ........................... 56
Toward Single-metal-ion Sensing by Förster Resonance
Energy Transfer.
By Jens U. Sutter, Alexander Macmillan, David J.S. Birch,
and Olaf J. Rolinski ........................................ 62
Fluorescence Interferometry: Principles and Applications
in Biology.
By Alberto Bilenca, Jing Cao, Max Colice, Aydogan Ozcan,
Brett Bouma, Laurel Raftery, and Guillcrmo Tearney .......... 68
Acoustically Levitated Droplets: A Contactless Sampling
Method for Fluorescence Studies.
By Jork Leiterer, Markus Grabolle, Knut Rurack, Ute
Resch-Genger, Jan Ziegler, Thomas Nann, and Ulrich Panne .... 78
Structural Effects of Biologically Relevant Rhodamines on
Spectroscopy of Fluorescence Fluctuations.
By José А. В. Ferreira ...................................... 85
Part III. Fluorescence Imaging
Dextrin-Microencapsulated Porphyrin: Luminescent Properties.
By Priscilla Paiva Luz, Claudio Roberto Neri,
and Osvaldo Antonio Serra ................................... 91
Lanthanide Bimetallic Hclicates for in Vitro
Imaging and Sensing.
By Jean-Claude G. Bünzli, Anne-Sophie Chauvin,
Caroline D.B. Vandevyver, Song Bo, and Steve Comby .......... 97
Time-Gated Luminescence Microscopy.
By Russell E. Connally and James A. Piper .................. 106
Time-resolved Microspectrofluorometry and Fluorescence
Imaging Techniques: Study of Porphyrin-mediated Cellular
Uptake of Oligonucleotides.
By Petr Praus, Eva Kočišova, Peter Mojzeš, Josef
Štépánek, Olivier Seksek, Franck Sureau,
and Pierre-Yves Turpin ..................................... 117
Part IV. Fluorescence Probes and Labels
Fluorescent DNA Base Modifications and Substitutes:
Multiple Fluorophore Labeling and the DETEQ Concept.
By Hans-Achim Wagenknecht .................................. 122
Fluorescent Probes for Chemical Transformations on the
Single-Molecule Level.
By Gregor Jung, Alexander Schmitt, Michaela Jacob, and
Babette Hinkeldey .......................................... 131
Luminescent Lanthanide Complexes as Probes for the
Determination of Enzyme Activities.
By Corinna M. Spangler, Christian Spangler, and Michael
Scháerling ................................................. 138
Which Are You Watching, an Individual Reactive Oxygen
Species or Total Oxidative Stress?
By Hatsuo Maeda ............................................ 149
Probing of Cosolvents in Polymer Latex Materials by Using
Solvatochromic Fluorescence.
By Albert M. Brouwer, Tanzeela N. Raja, Koen Biemans,
Tijs Nabuurs, and Ronald Tennebroek ........................ 157
Red/Near-infrared Boron Dipyrromcthene Dyes as Strongly
Emitting Fluorophores.
By Ana B. Descalzo, Hai-Jun Xu, Zhen Shen, and
Knut Rurack ................................................ 164
Sensitive Terbium Probes for Luminescent Determination
of both Alkaline Phosphatase and Codeine Phosphate.
By Axel Duerkop, Darya Aleksandrova, Yuliya
Scripinets, Alia Yegorova, and Ekateryna Vityukova ......... 172
Fluorescent Probes and Labels for Biomedical Applications.
By Leonid Patsenker, Anatoliy Tatarets, Olga Kolosova,
Olena Obukhova, Yevgeniy Povrozin, Iryna Fedyunyayeva,
Inna Yermolenko, and Ewald Terpetschnig .................... 179
Part V. Fluorescence Micro- and Nanoparticles
Photon Upconversion in Homogeneous Fluorescence-based
Bioanalytical Assays.
By Tero Soukka, Terhi Rantanen, and Katri Kuningas ......... 188
Processing and Characterization of Gold Nanoparticles
for Use in Plasmon Probe Spectroscopy and Microscopy
of Biosystems.
By Yu Chen, Jon A. Preece, and Richard E. Palmer ........... 201
Multifunctionalized Biocompatible Microspheres for Sensing.
By Rosario M. Sánchez-Martín, Lois Alexander, and
Mark Bradley ............................................... 207
Fluorescent Silica Nanoparticles.
By Heike Mader, Xiaohua Li, Sayed Saleh, Martin Link,
Peter Kele, and Otto S. Wolfbeis ........................... 218
Thermally Activated Delayed Fluorescence in Fullerenes.
By Carlos Baleizão and Mário N. Berberan-Santos ............ 224
Stability and Fluorescence Quantum Yield of CdSe - ZnS
Quantum Dots — Influence of the Thickness of the ZnS Shell.
By Markus Grabolle, Jan Ziegler, Alexei Merkulov,
Thomas Nann, and Ute Resch-Genger .......................... 235
Size Distributions of Cadmium Sulfide Nanoparticles
Obtained from Templating Methods.
By Paulo J.G. Coutinho, Candido A.G. Mendes, and
Teresa S.V. Reis ........................................... 242
Excitation Energy Transfer and Trapping in Dye-Loaded Solid
Particles.
By Hernán В. Rodríguez and Enrique San Román ................ 247
Characterization of Water-soluble Luminescent Quantum Dots
by Fluorescence Correlation Spectroscopy.
By Chaoqing Dong, Xiangyi Huang, and Jicun Ren ............. 253
Luminescent Amino-functionalized or Erbium-doped Silica
Spheres for Biological Applications.
By Francesco Enrichi ....................................... 262
Preparation and Characterization of Nanocrystalline
Zr02:Yb3+,Er3+ Up-conversion Phosphors.
By Iko Hyppänen, Jorma Hölsä, Jouko Kankare,
Mika Lastusaari, and Laura Pihlgren ........................ 267
Nano- and Microparticles of Organic Fluorescent Dyes:
Self-organization and Optical Properties.
By Suzanne Fery-Forgues, Mouhammad Abyan, and
Jean-Francois Lamere ....................................... 272
Part VI. Biophysical Fluorescence and Bioanalytical
Fluorescence
Flow Cytometric FRET Analysis of erbB Receptor Interaction
on a Gell-by-Cell Basis.
By Simone Diermeier-Daucher, Max Hasmann, and
Gero Brockhoff ............................................. 280
Toward Improved Biochips Based on Rolling Circle
Amplification—Influences of the Microenvironment
on the Fluorescence Properties of Labeled DNA
Oligonucleotides.
By Elke Mayer-Enthart, Julien Sialelli, Knut Rurack, Ute
Resch-Genger, Daniela Köster, and Harald Seitz ............. 287
Fluorescent Studies on Cooperative Binding of Cationic
Pheophorbide-a Derivative to Polyphosphate.
By Olga Ryazanova, Igor Voloshin, Igor Dubey, Larisa
Dubey, and Victor Zozulya .................................. 293
Effect of pH on Aqueous Phenylalanine Studied Using
a 265-nm Pulsed Light-emitting Diode.
By Alexander M. Macmillan, Colin D. McGuinness,
Kulwinder Sagoo, David McLoskey John C. Pickup,
and David J. S. Birch ...................................... 300
Ordered Self-assembly of Protonated Porphyrin Induced
by the Aqueous Environment of Biomimetic Systems.
By Suzana M. Andrade and Sílvia M.B. Costa ................. 305
Human Serum Albumin-flavonoid Interactions Monitored
by Means of Tryptophan Kinetics.
By Olaf J. Rolinski, Andrew Martin, and David J.S. Birch ... 314
Two-photon Excitation Fluorescence Bioassays.
By Pekka Hänninen, Jori Soukka, and Juhani T. Soini ........ 320
Index of Contributors ......................................... 327
The following companies contributed financially
to the conference:
Active Motif Chromeon
Edinburgh Instruments
Horiba-Jobin Yvon
Nikon
pco.imaging
PicoQuant
Tecan
ThermoFisher Scientific
Unilever
Zeiss
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